Review

human breast cancer cell line hs578t (ATCC)

ATCC is a verified supplier

ATCC manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

ATCC human breast cancer cell line hs578t
Human Breast Cancer Cell Line Hs578t, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 2321 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+breast+cancer+cell+lines+hs578t/Hs+578T/pm42020392-214-1-10
Average 97 stars, based on 2321 article reviews

human breast cancer cell line hs578t - by Bioz Stars, 2026-09

97/100 stars

Images

Multiple Displacement Amplification:

Article Title: Innate immune evasion revealed in a colorectal zebrafish xenograft model
Article Snippet: Validation data for Flow Cytometry antibodies is available on the homepage of the manufacterer Biolegend, eBiosceince and Thermo Scientific. .. Human breast cancer cell lines Hs578T, MDA-MB-231 and MDA-MB-468 were derived from American Type Culture Collection and kindly provided by Monica Bettencourt Dias'Lab (Instituto Gulbenkian de Ciência). .. Human colorectal cancer cell lines SW480, SW620 and HT29 were purchased from ATCC, whereas HCT116 and Hke3 isogenic cell lines were derived from American Type Culture Collection and kindly provided by Ângela Relógio (Charité Medical University of Berlin).

Article Title: MicroRNA-570 targets the HSP chaperone network, increases proteotoxic stress and inhibits mammary tumor cell migration
Article Snippet: The co-expression correlation dataset of breast tumor (960 samples) from TCGA was analyzed using cBioportal . .. Human embryonic kidney cell line HEK293T, human non-tumorigenic epithelial cell line MCF10A and human breast cancer cell lines Hs578T, MDA-MB231 and SKBR3 and HeLa cell line were obtained and cultured as recommended by American Type Culture Collection (ATCC, Gaithersburg, MD, USA). ..

Article Title: Anticancer Activity of Tubulosine through Suppression of Interleukin-6-Induced Janus Kinase 2/Signal Transducer and Activation of Transcription 3 Signaling
Article Snippet: The human mammary breast epithelial cell line MCF-10A was obtained from the American Type Culture Collection (Manassas, USA) and maintained in Dulbecco's Modified Eagle Medium (DMEM)/F-12 medium supplemented with 5% horse serum, 100 ng/mL cholera toxin, 20 ng/mL EGF, 0.5 mg/mL hydrocortisone, 10 μg/mL insulin, and 1% penicillin/streptomycin (HyClone, Pittsburgh, USA). .. The human breast cancer cell lines Hs578T, MCF-7, MDA-MB-231, and MDA-MB-468 were obtained from the American Type Culture Collection and maintained in DMEM, Eagle's Minimal Essential Medium, or Leibovitz's L-15 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. ..

Article Title: Chromene-based compounds, methods and uses thereof
Article Snippet: .. In an embodiment, three human breast cancer cell lines Hs578T, MDA-MB-468 and MCF-7, and a normal breast cell line MCF-10A, were obtained from ATCC (American Type Culture Collection). .. The cancer cell lines were cultured in Dulbecco's modified Eagle medium, 4.5 g/l glucose (DMEM, Gibco) supplemented with 10% heating inactivated Fetal Bovine Serum (FBS, Gibco) and 1% antibiotic solution (Penicillin-Streptomycin, Gibco).

Article Title: Hypoxia facilitates triple-negative breast cancer stem cells enrichment and stemness maintenance through oxidized ataxia telangiectasia mutated-induced one-carbon metabolism
Article Snippet: .. The human breast cancer cell lines Hs578T and MDA-MB-231 were acquired from the American Type Culture Collection. .. Hs578T cells were maintained in DMEM medium (Gibco, NY, United States), and MDA-MB-231 cells in RPMI-1640 medium (Gibco, NY, United States), both supplemented with 10% fetal bovine serum (Gibco, NY, United States), 100 U/mL penicillin, and 100 μg/mL streptomycin.

Article Title: MicroRNA-570 targets the HSP chaperone network, increases proteotoxic stress and inhibits mammary tumor cell migration.
Article Snippet: The dynamic network of chaperone interactions known as the chaperome contributes significantly to the proteotoxic cell response and the malignant phenotype.. To bypass the inherent redundancy in the network, we have used a microRNA (mir) approach to target multiple members of the chaperome simultaneously.. We identified a potent microRNA, miR‐570 that could bind the 3′untranslated regions of multiple HSP mRNAs and inhibit HSP synthesis.

Article Title: Identifying and targeting key driver genes for collagen production within the 11q13/14 breast cancer amplicon
Article Snippet: Transfection reagents: P4HA3 (#L-008479-01-0005), SERPINH1 (#L-011230-00-0005) and DharmaFECT (#T-2001-01) were purchased from Dharmacon Reagents (Horizon). .. Human breast cancer cell lines HS578T, MDA-MB-436, MDA-MB-361 and ZR-75-1 were obtained from the ATCC (Manassas, Virginia). ..

Derivative Assay:

Article Title: Tumour-specific STING agonist synthesis via a two-component prodrug system.
Article Snippet: Engineered THP-1 Lucia ISG cells were from InvivoGen (cat. thpl-isg). .. Human breast cancer cell lines Hs578T, were derived from American Type Culture Collection and kindly provided by Monica Bettencourt Dias'Lab (Instituto Gulbenkian de Ciência). .. MC38 was purchased from Kerafast (cat. ENH204-FP).

Cell Culture:

Image Search Results

Hypoxia promotes enrichment and DNA damage-independent oxidative activation of ataxia telangiectasia mutated. A: Flow cytometry analysis of CD44+/CD24- cell populations in Hs578T and MDA-MB-231 cells after exposure to hypoxia or normoxia; B: Quantitative reverse transcriptase polymerase chain reaction analysis of cancer stem cells-associated genes ( c-Myc , octamer-binding protein 4, Kruppel-like factor 4, sex-determining region Y-box 2, NANOG ) in mammosphere cultures under hypoxia; C-E: Western blot analysis of phosphorylated ataxia telangiectasia mutated, γH2AX, and 53BP1 in Hs578T and MDA-MB-231 cancer stem cells under normoxia, hypoxia, or H 2 O 2 treatment. Data were presented as mean ± SD ( n = 3). a P < 0.05; b P < 0.01; ns: not significant. KLF4: Kruppel-like factor 4; SOX2: Sex-determining region Y-box 2; OCT4: Octamer-binding protein 4; p-ATM: Phosphorylated ataxia telangiectasia mutated.

Journal: World Journal of Stem Cells

Article Title: Hypoxia facilitates triple-negative breast cancer stem cells enrichment and stemness maintenance through oxidized ataxia telangiectasia mutated-induced one-carbon metabolism

doi: 10.4252/wjsc.v18.i1.112278

Figure Lengend Snippet: Hypoxia promotes enrichment and DNA damage-independent oxidative activation of ataxia telangiectasia mutated. A: Flow cytometry analysis of CD44+/CD24- cell populations in Hs578T and MDA-MB-231 cells after exposure to hypoxia or normoxia; B: Quantitative reverse transcriptase polymerase chain reaction analysis of cancer stem cells-associated genes ( c-Myc , octamer-binding protein 4, Kruppel-like factor 4, sex-determining region Y-box 2, NANOG ) in mammosphere cultures under hypoxia; C-E: Western blot analysis of phosphorylated ataxia telangiectasia mutated, γH2AX, and 53BP1 in Hs578T and MDA-MB-231 cancer stem cells under normoxia, hypoxia, or H 2 O 2 treatment. Data were presented as mean ± SD ( n = 3). a P < 0.05; b P < 0.01; ns: not significant. KLF4: Kruppel-like factor 4; SOX2: Sex-determining region Y-box 2; OCT4: Octamer-binding protein 4; p-ATM: Phosphorylated ataxia telangiectasia mutated.

Article Snippet: The human breast cancer cell lines Hs578T and MDA-MB-231 were acquired from the American Type Culture Collection.

Techniques: Activation Assay, Flow Cytometry, Reverse Transcription, Polymerase Chain Reaction, Binding Assay, Western Blot

Oxidized ataxia telangiectasia mutated promotes serine hydroxymethyltransferase 2 and methylenetetrahydrofolate dehydrogenase 2 expression through c-Myc. A and B: Western blot analysis of phosphorylated ataxia telangiectasia mutated, c-Myc, serine hydroxymethyltransferase 2 (SHMT2), and methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) in Hs578T and MDA-MB-231 cells after treatment with Ku60019 and ataxia telangiectasia mutated knockdown; C: Consensus c-Myc binding motif; D: Schematic representation of predicted c-Myc binding sites in the human MTHFD2 and SHMT2 promoter regions; E: Luciferase assay showed SHMT2 and MTHFD2 relative luciferase activity; F: Representative chromatin immunoprecipitation (ChIP)-polymerase chain reaction (PCR) showing c-Myc occupancy at the MTHFD2 and SHMT2 promoters; input and immunoglobulin G served as controls; G and H: ChIP-quantitative PCR analysis demonstrating c-Myc enrichment at the MTHFD2 (G) and SHMT2 (H) promoters in Hs578T and MDA-MB-231 cells. ChIP-quantitative PCR enrichment expressed as % input relative to immunoglobulin G. Data were presented as mean ± SD ( n = 3). a P < 0.05; b P < 0.01. p-ATM: Phosphorylated ataxia telangiectasia mutated; MTHFD2: Methylenetetrahydrofolate dehydrogenase 2; SHMT2: Serine hydroxymethyltransferase 2; IgG: Immunoglobulin G.

Journal: World Journal of Stem Cells

Article Title: Hypoxia facilitates triple-negative breast cancer stem cells enrichment and stemness maintenance through oxidized ataxia telangiectasia mutated-induced one-carbon metabolism

doi: 10.4252/wjsc.v18.i1.112278

Figure Lengend Snippet: Oxidized ataxia telangiectasia mutated promotes serine hydroxymethyltransferase 2 and methylenetetrahydrofolate dehydrogenase 2 expression through c-Myc. A and B: Western blot analysis of phosphorylated ataxia telangiectasia mutated, c-Myc, serine hydroxymethyltransferase 2 (SHMT2), and methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) in Hs578T and MDA-MB-231 cells after treatment with Ku60019 and ataxia telangiectasia mutated knockdown; C: Consensus c-Myc binding motif; D: Schematic representation of predicted c-Myc binding sites in the human MTHFD2 and SHMT2 promoter regions; E: Luciferase assay showed SHMT2 and MTHFD2 relative luciferase activity; F: Representative chromatin immunoprecipitation (ChIP)-polymerase chain reaction (PCR) showing c-Myc occupancy at the MTHFD2 and SHMT2 promoters; input and immunoglobulin G served as controls; G and H: ChIP-quantitative PCR analysis demonstrating c-Myc enrichment at the MTHFD2 (G) and SHMT2 (H) promoters in Hs578T and MDA-MB-231 cells. ChIP-quantitative PCR enrichment expressed as % input relative to immunoglobulin G. Data were presented as mean ± SD ( n = 3). a P < 0.05; b P < 0.01. p-ATM: Phosphorylated ataxia telangiectasia mutated; MTHFD2: Methylenetetrahydrofolate dehydrogenase 2; SHMT2: Serine hydroxymethyltransferase 2; IgG: Immunoglobulin G.

Article Snippet: The human breast cancer cell lines Hs578T and MDA-MB-231 were acquired from the American Type Culture Collection.

Techniques: Expressing, Western Blot, Knockdown, Binding Assay, Luciferase, Activity Assay, Chromatin Immunoprecipitation, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

Serine hydroxymethyltransferase 2 and methylenetetrahydrofolate dehydrogenase 2 promote cancer stem cells enrichment and stemness maintenance in triple-negative breast cancer. A: Effects of serine hydroxymethyltransferase 2 knockdown on mammosphere formation, size, and number in Hs578T and MDA-MB-231 cancer stem cells; B: Effects of methylenetetrahydrofolate dehydrogenase 2 knockdown on mammosphere formation, size, and number; C and D: Western blot analysis of stemness-associated proteins Kruppel-like factor 4 and sex-determining region Y-box 2 after serine hydroxymethyltransferase 2 or methylenetetrahydrofolate dehydrogenase 2 knockdown. Scale bar: 200 μm. Data were presented as mean ± SD ( n = 3). a P < 0.05. MTHFD2: Methylenetetrahydrofolate dehydrogenase 2; SHMT2: Serine hydroxymethyltransferase 2; KLF4: Kruppel-like factor 4; SOX2: Sex-determining region Y-box 2.

Journal: World Journal of Stem Cells

Article Title: Hypoxia facilitates triple-negative breast cancer stem cells enrichment and stemness maintenance through oxidized ataxia telangiectasia mutated-induced one-carbon metabolism

doi: 10.4252/wjsc.v18.i1.112278

Figure Lengend Snippet: Serine hydroxymethyltransferase 2 and methylenetetrahydrofolate dehydrogenase 2 promote cancer stem cells enrichment and stemness maintenance in triple-negative breast cancer. A: Effects of serine hydroxymethyltransferase 2 knockdown on mammosphere formation, size, and number in Hs578T and MDA-MB-231 cancer stem cells; B: Effects of methylenetetrahydrofolate dehydrogenase 2 knockdown on mammosphere formation, size, and number; C and D: Western blot analysis of stemness-associated proteins Kruppel-like factor 4 and sex-determining region Y-box 2 after serine hydroxymethyltransferase 2 or methylenetetrahydrofolate dehydrogenase 2 knockdown. Scale bar: 200 μm. Data were presented as mean ± SD ( n = 3). a P < 0.05. MTHFD2: Methylenetetrahydrofolate dehydrogenase 2; SHMT2: Serine hydroxymethyltransferase 2; KLF4: Kruppel-like factor 4; SOX2: Sex-determining region Y-box 2.

Article Snippet: The human breast cancer cell lines Hs578T and MDA-MB-231 were acquired from the American Type Culture Collection.

Techniques: Knockdown, Western Blot

Fig. 2. NID1 downregulated cell migration and EMT marker expression. (A) Wound-healing assay of the MDA-MB-231 and Hs578T NID1-overexpressing cell lines. The healed rate of each wound area was measured at 24 h intervals. (B–C) (B) Transwell migration and (C) invasion assays were performed with the MDA- MB-231 and Hs578T NID1-overexpressing cell lines. (D) Immunofluorescence analysis showed decreased expression of the mesenchymal marker vimentin (scale bar = 100 μm). (E) Western blot analysis of total cell lysate with the indicated antibodies showed decreased expression of EMT marker proteins. (F) RT-qPCR analysis showing the mRNA expression of EMT markers in the indicated cell lines. The values were normalized to the GAPDH level. The data in (A, B, C, and F) are presented as mean ± SD. P values in (A, B, C, and F) were calculated using Student’s t test (* P < 0.05; ** P < 0.005; *** P < 0.0005).

Journal: Scientific reports

Article Title: Nidogen-1 suppresses cell proliferation, migration, and glycolysis via integrin β1-mediated HIF-1α downregulation in triple-negative breast cancer.

doi: 10.1038/s41598-024-84880-5

Figure Lengend Snippet: Fig. 2. NID1 downregulated cell migration and EMT marker expression. (A) Wound-healing assay of the MDA-MB-231 and Hs578T NID1-overexpressing cell lines. The healed rate of each wound area was measured at 24 h intervals. (B–C) (B) Transwell migration and (C) invasion assays were performed with the MDA- MB-231 and Hs578T NID1-overexpressing cell lines. (D) Immunofluorescence analysis showed decreased expression of the mesenchymal marker vimentin (scale bar = 100 μm). (E) Western blot analysis of total cell lysate with the indicated antibodies showed decreased expression of EMT marker proteins. (F) RT-qPCR analysis showing the mRNA expression of EMT markers in the indicated cell lines. The values were normalized to the GAPDH level. The data in (A, B, C, and F) are presented as mean ± SD. P values in (A, B, C, and F) were calculated using Student’s t test (* P < 0.05; ** P < 0.005; *** P < 0.0005).

Article Snippet: The MDA-MB-231 and Hs578T human breast cancer cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Migration, Marker, Expressing, Wound Healing Assay, Immunofluorescence, Western Blot, Quantitative RT-PCR

Fig. 5. NID1 suppressed the FAK/Src/NF-κB p65 signaling axis via integrin β1 downregulation. (A) Western blot analysis of total cell lysates with the indicated antibodies. β-actin was used as the loading control. (B) RT- qPCR analysis showing the mRNA expression of ITGB1 in the indicated cell lines. Values were normalized to GAPDH. (C–D) Cycloheximide (CHX) chase assays were performed to measure the degradation of integrin β1 in the (C) MDA-MB-231 and (D) Hs578T NID1-overexpressing cell lines. Cells were treated with CHX (40 μg/ ml) and harvested at the indicated times. (E) To detect ubiquitinated integrin β1, an immunoprecipitation analysis was performed with anti-integrin β1 antibody after a 24-h incubation with MG132 (10 μM). (F) Western blot analysis of total cell lysates with the indicated antibodies; GAPDH was used as the loading control. (G) Dual luciferase assays were performed using NF-κB-luc constructs. pRL-CMV was used as the internal control. (H) Kaplan–Meier survival analysis of breast cancer patients based on NID1 expression levels. (I) A schematic description of the NID1-mediated signaling pathway in breast cancer cells. Data in (B, C, D, and G) are presented as mean ± SD. P values in (B, C, D, and G) were calculated using Student’s t test; in H, they were calculated with the log-rank test (*P < 0.05; **P < 0.005; ***P < 0.0005).

Journal: Scientific reports

Article Title: Nidogen-1 suppresses cell proliferation, migration, and glycolysis via integrin β1-mediated HIF-1α downregulation in triple-negative breast cancer.

doi: 10.1038/s41598-024-84880-5

Figure Lengend Snippet: Fig. 5. NID1 suppressed the FAK/Src/NF-κB p65 signaling axis via integrin β1 downregulation. (A) Western blot analysis of total cell lysates with the indicated antibodies. β-actin was used as the loading control. (B) RT- qPCR analysis showing the mRNA expression of ITGB1 in the indicated cell lines. Values were normalized to GAPDH. (C–D) Cycloheximide (CHX) chase assays were performed to measure the degradation of integrin β1 in the (C) MDA-MB-231 and (D) Hs578T NID1-overexpressing cell lines. Cells were treated with CHX (40 μg/ ml) and harvested at the indicated times. (E) To detect ubiquitinated integrin β1, an immunoprecipitation analysis was performed with anti-integrin β1 antibody after a 24-h incubation with MG132 (10 μM). (F) Western blot analysis of total cell lysates with the indicated antibodies; GAPDH was used as the loading control. (G) Dual luciferase assays were performed using NF-κB-luc constructs. pRL-CMV was used as the internal control. (H) Kaplan–Meier survival analysis of breast cancer patients based on NID1 expression levels. (I) A schematic description of the NID1-mediated signaling pathway in breast cancer cells. Data in (B, C, D, and G) are presented as mean ± SD. P values in (B, C, D, and G) were calculated using Student’s t test; in H, they were calculated with the log-rank test (*P < 0.05; **P < 0.005; ***P < 0.0005).

Article Snippet: The MDA-MB-231 and Hs578T human breast cancer cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Western Blot, Control, Quantitative RT-PCR, Expressing, Immunoprecipitation, Incubation, Luciferase, Construct

Review

human breast cancer cell line hs578t (ATCC)

Structured Review

Images

Related Articles

Multiple Displacement Amplification:

Derivative Assay:

Cell Culture:

Similar Products

Image Search Results